D2560

DNase test agar with toluidine blue

NutriSelect^® Basic, powder, for bacteria, for fungi, pack of 500 g, suitable for microbiology

• Synonym(s): DNase agar (with TBO),, DNase test agar withtoluidine blue O (TBO), Deoxyribonuclease test agar with toluidine blue
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Basic
• Form: powder
• Suitability: bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)
• Application(s): food and beverages, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 100
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbe id | specific enzyme detection: suitable, microbiological culture: suitable
• color: blue
• final pH: 7.3±0.2 (25 °C)
• application(s): food and beverages, microbiology
• suitability: bacteria (deoxyribonuclease activity), fungi, nonselective and differential for Serratia spp., nonselective and differential for Staphylococcus spp., nonselective and differential for Streptococcus spp., nonselective and differential for molds (General Media), nonselective and differential for yeasts (General Media)

Description

General description

The DNase Test Agar with Toluidine Blue is a specialized microbiological medium used to detect deoxyribonuclease (DNase) activity in bacteria and fungi. It incorporates toluidine blue as an indicator, which produces a distinct color change when DNase activity is present. This medium is particularly effective for differentiating and identifying nonpigmented Serratia species. Tryptose in the formulation provides essential nutrients, while DNase activity depolymerizes DNA, triggering a bright pink reaction due to the metachromatic properties of toluidine blue. However, some Staphylococcus strains may experience growth inhibition on this medium because of the toluidine blue. Additional confirmatory tests are recommended for accurate identification.

Application

DNase Test Agar with Toluidine Blue is recommended for the detection of deoxyribonuclease activity of bacteria and fungi, especially for the identification of pathogenic Staphylococci. This media can be used in food and beverage testing, clinical microbiology, and general microbiological research.

Features and Benefits

NutriSelect^® Basic is a powdered culture media offering

• Qualitycontrol with growth promotion test according to industry standards
• Qualityassurance acc. to ISO 9001
• Cost-efficient powderedmedia

Preparation Note

Suspend 42.1 g of DNase Test Agar with Toluidine Blue in 1000 ml of distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs. pressure (118°C to 121°C) for 15 minutes. Cool to 45°C and pour into sterile petri plates.

Other Notes

Ingredients (g/L)
Tryptose, 20.00
Deoxyribonucleic acid (DNA), 2.00
Sodium chloride, 5.00
Toluidine blue, 0.10
Agar, 15.00

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)