C2734

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

• Synonym(s): 5-TAMRA
• Empirical Formula (Hill Notation): C₂₅H₂₂N₂O₅
• CAS Number: 91809-66-4
• Molecular Weight: 430.45
• UNSPSC Code: 12171500
• NACRES: NA.47
• MDL number: MFCD01073638

Properties

• Product Name: 5-Carboxytetramethylrhodamine,
• assay: ≥95% (HPLC)
• Quality Segment: 100
• form: solid
• solubility: water: slightly soluble
• ε (extinction coefficient): ≥85000-97000 at 539-545 nm in methanol
• fluorescence: λ_ex ~543 nm; λ_em ~570 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C
• InChI: 1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
• InChI key: YMZMTOFQCVHHFB-UHFFFAOYSA-N

Description

General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)