96961

m-FC-Agar

NutriSelect^® Plus, according to APHA, powder, selective and differential for coliforms, pack of 500 g, suitable for microbiology

• Synonym(s): Membrane Fecal Coliform Agar, m-Fecal Coliform Agar
• NACRES: NA.85
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Plus
• Agency: according to APHA
• Form: powder
• Suitability: selective and differential for Escherichia coli, selective and differential for coliforms
• Application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring, microbiology

Properties

• Quality Segment: 200
• agency: according to APHA
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar-agar, 15 g/L , bile salts, 1.5 g/L , lactose, 12 g/L , methyl blue, 0.1 g/L , proteose peptone, 5 g/L , sodium chloride, 5 g/L , tryptose, 10 g/L , yeast extract, 3 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: beige
• final pH: 7.4±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring, microbiology
• suitability: selective and differential for Escherichia coli, selective and differential for coliforms

Description

General description

m-FC Agar is a selective medium designed for the detection and enumeration of fecal coliform bacteria in water samples. It is widely used in environmental microbiology for water quality testing. The medium contains selective agents that inhibit the growth of non-target organisms while promoting the growth of fecal coliforms. Proteose peptone, Tryptose, and Yeast extract act as sources of carbon, nitrogen, minerals, vitamins, and other essential growth nutrients. Lactose is a fermentable carbohydrate. mFC Agar contains Bile salts, which inhibit the accompanying gram-positive bacteria. Methyl blue is a triphenylmethane dye that suppresses the growth of many gram-positive bacteria. The indicator system is based on the color change of methyl blue and rosolic acid. The high incubation temperature makes the test more selective.

Application

m-FC Agar is used for the detection and enumeration of fecal coliforms using membrane filter technique. This medium is suitable for detecting fecal coliforms in water samples, ensuring compliance with environmental and public health standards.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Dissolve 52 g in 1 litre distilled water and heat to boiling to dissolve completely. Add 10 ml of a 1 % solution of rosolic acid (Cat. No. 861324) in 0.2 N NaOH. Continue heating for 1 minute with frequent agitation. Do not autoclave! Cool to 45-50°C. Dispense 4 ml amounts into Petri dishes (50-60mm) and allow to solidify.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)