91365

Reinforced Clostridial Agar

NutriSelect^® Plus, powder, nonselective for Clostridium spp., pkg of 500 g, suitable for microbiology

• Synonym(s): RCA, RCM Agar, RC Agar, RCM
• NACRES: NA.85
• UNSPSC Code: 41171607
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: nonselective for Bacteroides spp., nonselective for Clostridium spp.
• Application(s): agriculture; bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; veterinary, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 13.5 g/L , beef extract, 10 g/L , casein enzymatic hydrolysate, 10 g/L , L-cysteine hydrochloride, 0.5 g/L , dextrose, 5 g/L , sodium acetate, 3 g/L , sodium chloride, 5 g/L , starch soluble, 1 g/L , yeast extract, 3 g/L
• packaging: pkg of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 6.8±0.2 (25 °C)
• application(s): agriculture; bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; veterinary, microbiology
• suitability: nonselective for Bacteroides spp., nonselective for Clostridium spp.

Description

General description

Reinforced Clostridial Agar (RCA) is a powdered microbiological culture medium designed for the cultivation and enumeration of Clostridia. It contains essential nutrients such as beef extract, casein enzymatic hydrolysate, yeast extract, and dextrose, which provide nitrogen, vitamins, amino acids, and carbon for microbial growth. Sodium acetate acts as a buffering agent, while L-cysteine hydrochloride serves as a reducing agent to support anaerobic conditions. The medium has a final pH of 6.8 ± 0.2 at 25°C and is non-selective for Clostridium spp. and Bacteroides spp.

Application

Reinforced Clostridial Agar is widely used in clinical testing, food and beverage microbiology for the cultivation and enumeration of Clostridia. It is suitable for anaerobic bacteria and is commonly employed in bioburden testing, agriculture, and personal care product microbiology.

Features and Benefits

NutriSelectn^® Plus is a powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Suspend 51 g of Reinforced Clostridial Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 10 lbs. Pressure (115°C) for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)