64198

Nocive Brewers Bacteria Agar Base, modified

NutriSelect^® Plus, powder, selective for Lactobacillus spp., for Pediococcus spp., pack of 500 g, suitable for microbiology

• Synonym(s): Detection Agar for bacterial beer spoiler, Pedi-Lacto Selective Beer Agar
• NACRES: NA.85
• UNSPSC Code: 41171609
• Manufacturer/tradename: NutriSelect^® Plus
• Form: powder
• Suitability: Leuconostoc spp., Pediococcus spp., selective for Lactobacillus spp., selective for Lactococcus spp.
• Application(s): bioburden testing; food and beverages, microbiology

Properties

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: bacteriological Agar, 15 g/L , beef extract, 2 g/L , chlorophenol red, 0.07 g/L , L-cysteine hydrochloride, 0.2 g/L , dextrose, 15 g/L , disodium phosphate, 2 g/L , L-malic acid, 0.5 g/mL , maltose, 15 g/L , pancreatic digest of casein, 5 g/L , polysorbate 80, 0.5 g/L , potassium acetate, 6 g/L , yeast extract, 5 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• color: gray-brown
• final pH: 5.8±0.2 (25 °C)
• application(s): bioburden testing; food and beverages, microbiology
• suitability: Leuconostoc spp., Pediococcus spp., selective for Lactobacillus spp., selective for Lactococcus spp.

Description

General description

Nocive Brewers Bacteria Agar is a selective medium designed for thedetection of beer-spoiling microorganisms. Originally developed in Germany byBack and Dachs, the medium was later modified byNishikawa and Kohgo to reduce its inhibitory effects on beer-spoilingorganisms. Pancreatic digest of casein, yeast extract, and beef extract providesessential nitrogenous compounds, vitamins, and nutrients required for thegrowth of spoilage bacteria. Dextrose and maltose serve as fermentable sugars,while chlorophenol red acts as an indicator for acid production. Polysorbate 80neutralizes phenols, hexachlorophene, and formalin, and potassium acetatereplaces sodium acetate to make the medium less inhibitory. L-cysteinehydrochloride functions as a reducing agent, and L-malic acid supports thegrowth of lactic acid bacteria as a beneficial metabolite. Disodium phosphateserves as a buffering agent, while the low pH optimizes the growth of lacticacid bacteria and simultaneously inhibits other organisms. Agar is used as thesolidifying agent.

Application

NociveBrewers Bacteria Agar is specifically designed for the detection and isolationof beer-spoiling microorganisms, including lactic acid bacteria and otherspoilage organisms that can compromise the quality and stability of beer. Themedium is used to monitor contamination during various stages of beerproduction, ensuring product integrity and preventing spoilage.

Features and Benefits

Features:

• High-Quality Standards: NutriSelect^® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
• Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
• Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
• Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.

Benefits:

• Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
• Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment. ·
• Extended Shelf Life: Products have a prolonged shelf life of up to five years.
• High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
• Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Suspend 66,3 grams of the medium in 500 ml of distilled water and 500 ml of beer without gas. Mix well. Heat for one minute with frequent agitation until the medium is completelydissolved. Sterilize in autoclave at 121°C for 15 minutes. Cool at 50°C and pour into Petri dishes.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable