For Enzymatic Activity testing, this product is reconstituted at 1000 units/mL in 60 mM KH2PO4 containing 17 mM NaCl, pH 7.0. This enzyme is active over a broad pH range (6.0–9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02–0.100 M) than at pH 9.2 (0.01–0.06 M). Other sources report, in crystallization studies, Sodium Acetate buffer, pH 4.5 – 4.7 has been used to prepare solutions at a concentration range of 100 – 250 mg/mL. Please see the link below to review a publication that may be of interest:
https://pmc.ncbi.nlm.nih.gov/articles/PMC12917727/
62970
Lysozyme from chicken egg white
dialyzed, lyophilized, powder, ~100000 U/mg
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
235-747-3
MDL number:
EC Number:
Specific activity:
~100000 U/mg
Biological source:
chicken egg white
Pricing and availability is not currently available.
biological source
chicken egg white
Quality Segment
form
powder
quality
dialyzed, lyophilized
specific activity
~100000 U/mg
mol wt
single-chain 14.3 kDa, Mr ~14600
technique(s)
cell based assay: suitable
input
bacteria and yeast sample(s)
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
2-8°C
Gene Information
chicken ... LYZ(396218)
Application
Lysozyme from chicken egg white has been used:
- as a standard in small-angle neutron scattering experiments and infrared absorbance spectroscopy
- as a constituent of cell lysis buffer
- in protein crystallization experiments
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Other Notes
1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)
Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls.
1 of 1
This Item | |||
|---|---|---|---|
| description dialyzed, lyophilized, powder, ~100000 U/mg | description aseptically filled | description powder (crystalline), 70000-140000 U/mg | description powder or granules, ≥39,000 units/mg protein |
| biological source chicken egg white | biological source - | biological source chicken egg white | biological source chicken egg white |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| specific activity ~100000 U/mg | specific activity - | specific activity 70000-140000 U/mg | specific activity ≥39,000 units/mg protein |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) |
| form powder | form powder | form powder (crystalline) | form powder or granules |
| suitability suitable for cell lysis | suitability - | suitability suitable for cell lysis | suitability suitable for cell lysis |
Still not finding the right product?
Explore all of our products under Lysozyme from chicken egg white
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
What is the best working buffer for this enzyme? And what is the maximal concentration it can be resuspended?
1 answer-
Helpful?
-
-
can it be resuspended in water?
1 answer-
Yes, this product can be reconstituted in water at a concentration of 10 mg/ml.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-