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Merck

39441

Nitrate Reagent B

suitable for microbiology

Synonym(s):

Sulfanilic acid solution, 4-Aminobenzenesulfonic acid

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About This Item

CAS Number:
121-57-3
NACRES:
NA.85
PubChem Substance ID:
329755871
UNSPSC Code:
41171621
MDL number:
MFCD00007886

Key Documents

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Product Name

Nitrate Reagent B, suitable for microbiology

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

shelf life

limited shelf life, expiry date on the label

composition

acetic acid (30%), 1000 mL
sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Enterobacter spp.
Escherichia coli
Neisseria spp.
anaerobic bacteria
coliforms

Quality Level

100

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1 of 4

This Item
PHR141925191786090
Nitrate Reagent B suitable for microbiology

Millipore

39441

Nitrate Reagent B

Sulfanilic Acid Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1419

Sulfanilic Acid

Sulfanilic acid ACS reagent, 99%

Sigma-Aldrich

251917

Sulfanilic acid

Sulfanilic acid puriss. p.a., ≥99.0% (T)

Sigma-Aldrich

86090

Sulfanilic acid

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

composition

acetic acid (30%), 1000 mL

composition

-

composition

-

composition

-

technique(s)

microbe id | metabolite detection: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

application(s)

pharmaceutical (small molecule)

application(s)

-

application(s)

-

suitability

Enterobacter spp.

suitability

-

suitability

-

suitability

-

Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6942
BCCJ0564
BCCD0792
BCBV9795

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Han Ming Gan et al.
Microbiology (Reading, England), 158(Pt 8), 1933-1941 (2012-05-23)
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The
Han Ming Gan et al.
Chemosphere, 82(4), 507-513 (2010-11-26)
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100 mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation
Kwan Kim et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 100, 10-14 (2012-02-14)
We estimated the apparent size of the 'hot site' for surface-enhanced Raman scattering (SERS) located within the gaps between Pt nanoparticles and a flat Ag substrate. Initially, no Raman peaks were detected for 4-aminobenzenethiol (4-ABT) on a flat Ag substrate.
Han Ming Gan et al.
FEMS microbiology letters, 318(2), 108-114 (2011-02-18)
Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10,000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability
Yan-Qing Wang et al.
Huan jing ke xue= Huanjing kexue, 30(4), 1193-1198 (2009-06-24)
A 4-aminobenzenesulphonate-degrading strain W1 was isolated from the activated sludge of Dalian municipal wastewater treatment plant. Strain W1 was able to utilize 4-ABS as sole carbon and energy source under aerobic condition. It was identified as Pannonibacter sp. according to
View All Related Papers

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