TAQKB

KAPA Taq PCR Kit

• Synonym(s): PCR, Taq
• NACRES: NA.54
• UNSPSC Code: 41106300

Properties

• shelf life: ≤18 mo.
• feature: dNTPs included: no, hotstart: no
• packaging: pkg of 250 U (KK1014), pkg of 2500 U (BK1000), pkg of 500 U (KK1015), pkg of 5000 U (BK1002)
• manufacturer/tradename: Roche
• technique(s): PCR: suitable
• input: purified DNA
• storage temp.: −20°C

Description

Application

KAPA Taq PCR Kit has been used in:

• High throughput PCR
• Amplification of low copy DNA templates
• Multiplex PCR
• Specific amplification of complex templates
• RT-PCR
• random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
• Polymerase chain reaction (PCR)
• Genotyping

Biochem/physiol Actions

KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart^® DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 10⁵ nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

Features and Benefits

High performance:

• Improved sensitivity, specificity, and yields
• Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific product

Quick Notes:

• KAPA Taq DNA Polymerase can replace any commercial Taq DNA polymerase in an existing protocol.
• The final MgCl₂ concentration may need to be optimized to account for differences in buffer formulation.
• KAPA Taq Buffers contain MgCl₂ at a final concentration of 1.5 mM. Buffer A is recommended as first approach and for applications requiring high yields. Buffer B is recommended for applications where high sensitivity is required (e.g. when the template is limiting). Both buffers may be evaluated to determine the buffer most suitable for a specific application.
• The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C forshort-term use (up to 1 month). Return to -20°C for long term storage.

Analysis Note

Each batch of KAPA Taq DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230Assay). KAPA Taq Ready Mixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Kit Components Only

• KAPA Taq Standard or HotStart^® DNA Polymerase 5 U/μL
• 10X KAPA Taq Buffer A
• 10X KAPA Taq Buffer B
• MgCl₂ 25 mM

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash