EXPEXTKB

KAPA Express Extract

• Synonym(s): PCR, PCR extraction
• NACRES: NA.55
• UNSPSC Code: 41106300

Properties

• description: for extraction only
• usage: sufficient for 100 reactions (KK7101), sufficient for 250 reactions (KK7102), sufficient for 50 reactions (KK7100), sufficient for 500 reactions (KK7103)
• shelf life: ≤12 mo.
• packaging: pkg of 100 U (KK7100), pkg of 1000 U (KK7103), pkg of 200 U (KK7101), pkg of 500 U (KK7102)
• manufacturer/tradename: Roche
• technique(s): DNA extraction: suitable, PCR: suitable
• storage temp.: −20°C

Description

General description

KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.
The combination of KAPA Express Extract and KAPA2G Robust HotStart^® ReadyMix^™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.

Application

KAPA Express Extract is ideally suited for the extraction of polymerase chain reaction (PCR)-ready DNA from, but not limited to, the following sample types:

• Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood[1] collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples; biopsies)
• Animal samples (ear punches;[2][3] tail clippings;[4][1] hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
• Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples; embryo)
• Insects (crushed)
• Bird feathers (calamus fragments).
• Nosema species

Features and Benefits

Key Features:

• Rapid extraction protocol: PCR-ready DNA in 15 minutes.
• Versatility: single kit optimized for successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers.
• Single-tube reaction minimizes risk of contamination.
• Produces high amounts of genomic DNA of good quality.
• DNA extracted with KAPA Express Extract is compatible with any PCR system.

Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:

• Rapid DNA extraction and consistent downstream amplification of challenging sample types.

Routine DNA extraction from blood sample types:

• Efficient extraction from “Guthrie”cards, FTA^™ Elute cards, and FTA cards.

Quick Notes:

• Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
• Significantly reduced turnaround times and risk of sample loss and contamination.
• Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
• One extract typically yields a sufficient amount of template for 50–500 PCRs.
• DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
• KAPA2G Robust HotStart^® ReadyMix^™ is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
• No quantification of DNA required prior to PCR.

Preparation Note

Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.

Analysis Note

All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

FTA is a trademark of Qiagen Group

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

• KAPA Express Extract Enzyme 1 U/µL
• 10X KAPA Express Extract Buffer

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash