OP143

Anti-MDM2 (Ab-3) Mouse mAb (4B11)

liquid, clone 4B11, Calbiochem^®

• Synonym(s): Anti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Anti-Murine Double Minute Chromosome-2
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 4B11, monoclonal
• Species reactivity: mouse, human
• Application: —
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4B11, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: mouse, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (2 µg/mL, chemiluminescence); Immunofluorescence (1 µg/mL); Immunoprecipitation (1 µg/reaction); Paraffin Sections (2.5 µg/mL, heat pre-treatment required))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MDM2(4193) ; mouse ... Mdm2(17246)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells. May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2.

This Anti-MDM2 (Ab-3) Mouse mAb (4B11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-3).

Immunogen

Epitope: within amino acids 383-491

Human

full-length, recombinant human MDM2

Application

Immunoblotting (2 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A549 cells

Other Notes

Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.

May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3