NB20
Anti-AnkyrinG Mouse mAb (463)
liquid, ≥95% (SDS-PAGE), clone 463
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
463, monoclonal
Species reactivity:
rat, mouse, human, canine
Application:
—
Citations:
4
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
463, monoclonal
assay
≥95% (SDS-PAGE)
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, mouse, human, canine
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (1-2 µg/mL)
Immunoblotting (2-5 µg/mL)
Immunofluorescence (1-2 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ANK3(288)
General description
Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of AnkyrinG.
Recognizes the ~190 kDa ankyrinG protein in brain extracts. Reacts with all spliced forms of ankyrinG.Antibody Target Gene Symbol: ANK3 Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049 Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G) Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057) Mu Entrez ID: 11735 Rat Entrez ID: 361833
Immunogen
Human
a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrinG
Application
Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
Mouse or rat brain extract or unmyelinated axons
Mouse or rat brain extract or unmyelinated axons
Other Notes
AnkyrinG is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.
Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| NB20-100UG | 04055977209808 |