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MABT161

Sigma-Aldrich

Anti-Versican Antibody, clone 8D8.1

clone 8D8.1, from mouse

Synonym(s):

Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

8D8.1, monoclonal

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Vcan(13003)

General description

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

Specificity

This antibody recognizes the C-terminus of Veriscan.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human Veriscan.

Application

Detect Versican using this mouse monoclonal antibody, Anti-Versican Antibody, clone 8D8.1 validated for use in IHC & western blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human cerebellum, and mouse brain and embryonic skeletal muscle.

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

Evaluated by Immunohistochemistry in human thalamus tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.

Target description

372 kDa calculated. Uniprot describes three isoforms at 265 kDa, 182 kDa, and 74 kDa Versican is known to be secreted following valvular interstitual cell (VIC) injury and repair (Carthy, J., et al. (2012). Cardiovac. Pathol. 21(2):74-82.).

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human thalamus tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lulu Xu et al.
Molecular medicine reports, 13(6), 5005-5012 (2016-04-26)
The versican family is important in the modulation of inflammation, however, the role of versican V1 (V1) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying mechanisms remain to be elucidated. To investigate this, the present study performed experiments
Expression of a novel versican variant in dorsal root ganglia from spared nerve injury rats.
Oliver Bogen et al.
Molecular pain, 15, 1744806919874557-1744806919874557 (2019-08-21)
Suguru Yamasaki et al.
iScience, 25(1), 103657-103657 (2022-01-14)
Pluripotent stem cell (PSC)-derived retinal sheet transplanted in vivo can form structured photoreceptor layers, contact with host bipolar cells, and transmit light signals to host retinas. However, a major concern is the presence of graft bipolar cells that may impede host-graft

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