MABT134

Anti-VE-cadherin Antibody, clone BV6

clone BV6, from mouse

• Synonym(s): Cadherin-5, 7B4 Antigen
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: BV6, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: 17

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BV6, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001786
• UniProt accession no.: P33151
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CDH5(1003)

Description

General description

Human Vascular Endothelial (VE)-cadherin is a calcium-dependent adhesion molecule strictly located at cell-to-cell junctions. VE-cadherin is present in all types of endothelium (veins, arteries, capillary and large vessels). Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. VE-cadherin also associates with alpha-catenin forming a link to the cytoskeleton.

~120 kDa observed.
The calculated molecular weight is 82 kDa but it will run between ~90-140 kDa because this protein is glycosolated.

Immunogen

HUVEC cells

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected VE-cadherin in the cell-cell junctions of HUVEC cells.

Immunohistochemistry Analysis: A 1:10-1:20 dilution of a representative lot was used by an independent laboratory in paraffin-embedded tissue sections. Buffered formalin fixation is recommended with a fixation period of no longer than 12 hours. High heat antigen retrieval in citrate buffer (Cat. No. 21545) is also suggested. Antibody can also be used to label acetone-fixed cryostat sections or cells using a immunoperoxidase staining protocol (eg. IHC Select Detection Kit, Cat. No. DAB150)

Flow Cytometry Analysis: A representative lot was used in the presence of Ca2+. Note: Use PBS + 2-5mM EDTA for cell detachment.

Western Blot Analysis: Non-reducing conditions may be needed, Ca2+ required in buffer (2-5 mM).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-VE-cadherin Antibody, clone BV6 is validated for use in WB, IC, FC, IH(P) for the detection of VE-cadherin.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HUVEC cell lysate

Evaluated by Western Blot in HUVEC cell lysate.

Western Blot Anlaysis: A 1:2,000 dilution of this antibody detected VE-cadherin in HUVEC cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB1989

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable