MABF2155

Anti-Ricin A Chain Antibody, clone RAC18

clone RAC18, from mouse

• Synonym(s): RAC
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RAC18, monoclonal
• species reactivity: Ricinus communis
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; inhibition assay: suitable; neutralization: suitable
• isotype: IgG2aκ
• UniProt accession no.: Q0Q2G6
• target post-translational modification: unmodified

Description

General description

Ricin is a dimeric toxin derived from Ricinus communis (Caster bean). It is composed of a sugar-binding subunit (B chain) that attached to cell surface receptors and facilitate the entry of toxin into the target cell and a second subunit (A chain), whcih has glycosidase activity that inactivates ribosomes by depurinating a single adenosine residue from an exposed loop of the 28S ribosomal RNA. This leads to inhibition of protein synthesis in cells. The toxic effects of Ricin are attributed to the A chain. Clone RAC18 displays high avidity for intact ricin and inhibits its activity without affecting binding of ricin to cells. Immunization with RAC18 is shown to offer significant protection against ricin toxicity. (Ref.: Maddaloni, M., et al. (2004). J. Immunol. 172; 6221-6228).

Specificity

Clone RAC18 bind to Ricin A chain

Immunogen

Purified Ricin A chain.

Application

Anti-Ricin A Chain, clone RAC18, Cat. No. MABF2155, is a mouse monoclonal antibody that detects Ricin A chain and has been tested for use in ELISA, Inhibition Assay, and Neutralizing.

Neutralizing Analysis: A representative lot neutralized the activity of Ricin A chain. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

ELISA Analysis: A representative lot detected Ricin A chain in ELISA applications (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8; Simon, S., et. al. (2015). Toxins (Basel) 7(12):4967-86).

Inhibition Analysis: A representative lot inhibited the enzymatic activity of Ricin. (Maddaloni, M., et. al. (2004). J Immunol. 172(10):6221-8).

Research Category
Inflammation & Immunology

Quality

Evaluated by ELISA with Ricin A chain.

ELISA Analysis: Various dilutions of this antibody detected Ricin A chain in an indirect ELISA.

Target description

29.89 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.