MABF2097

Anti-Norovirus (MNV-1) Antibody, clone 5C4.10

clone 5C4.10, from mouse

• Synonym(s): MNV-1, Murine Norovirus
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 5C4.10, monoclonal
• Species reactivity: virus, norovirus
• Application: ELISA, WB
• Citations: 5

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5C4.10, monoclonal
• species reactivity: virus, norovirus
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable, western blot: suitable
• isotype: IgG2bκ
• target post-translational modification: unmodified

Description

General description

Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187 kDa polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59 kDa capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22 kDa basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. This monoclonal antibody, clone 5C4.10, recognizes the S domain portion of the viral capsid protein and is broadly reactive with pan-noroviruses of murine and human type. (Ref.: Kolawole AO, et al. (2017). mSphere 2: e00334-17; Kolawole, AO et al. (2014). J. Gen. Virol. 95(9); , 1958 1968; Royall, E., et al. (2015). J. Biol. Chem. 290(8); 4748-58; Wobus, CE et al. (2006). J. Virol. 80(11); 5104-5112).

~57 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Infectious and UV-inactivated murine norovirus-1.

Application

Anti-Norovirus (MNV-1), clone 5C4.10, Cat. No. MABF2097, is a mouse monoclonal antibody that detects murine Norovirus capsid protein and has been tested for use in ELISA and Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: A representative lot detected Norovirus (MNV-1) in Western Blotting applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

ELISA Analysis: A representative lot detected Norovirus (MNV-1) in ELISA applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68; Kolawole, A.O., et. al. (2017). mSphere. 2(5). pii: e00334-17).

Biochem/physiol Actions

Clone 5C4.10 specifically recognizes the S domain portion of capsid protein of murine Norovisrus.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in RAW 264.7 cells infected with Murine norovirus-1.CW3.

Western Blotting Analysis: 1 µg/mL of this antibody detected Norovirus (MNV-1) in RAW 264.7 cells infected with Murine norovirus-1.CW3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.