The species reactivity of this antibody is Eukaryote; it is expected to react with Drosophila. The Western blotting analysis of glutathione conjugated proteins was performed under non-reduced conditions. Interference in the presence of sodium dodecyl sulfate has not been investigated. Note that this antibody does not react with free glutathione.
MAB5310
Anti-Glutathione Antibody, clone D8
clone D8, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
D8, monoclonal
Species reactivity:
eukaryotes
Application:
WB
Citations:
14
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
D8, monoclonal
species reactivity
eukaryotes
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
isotype
IgG2a
shipped in
dry ice
target post-translational modification
unmodified
Immunogen
Glutathione-protein complexes.
Application
Anti-Glutathione Antibody, clone D8 is an antibody against Glutathione for use in WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Oxidative Stress
Oxidative Stress
Western blotting of glutatione conjugated proteins under non-reduced conditions: 1:500-1:1000; does not react with glutatione by itself.
Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with reduced or non-reduced glutathione on proteins only. Does not react with free glutathione.
Physical form
Format: Purified
Immunoglobulin purified by protein A affinity and presented as a liquid in PBS, pH 7.2 with 0.01% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone D8, Chemicon®, from mouse | description clone GS-6, Chemicon®, from mouse | description Chemicon®, from rabbit | description clone 4F5-D8, purified immunoglobulin, buffered aqueous solution |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| species reactivity eukaryotes | species reactivity human, rat, mouse, sheep | species reactivity eukaryotes | species reactivity human |
| clone D8, monoclonal | clone GS-6, monoclonal | clone polyclonal | clone 4F5-D8, monoclonal |
| technique(s) western blot: suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, ELISA: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable | technique(s) immunoprecipitation (IP): suitable, capture ELISA: suitable, indirect ELISA: suitable |
| isotype IgG2a | isotype IgG2a | isotype - | isotype IgG1κ |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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Hi, we would like to see if this antibody works for drosophila samples. Is it possible to order a small samples? In addition, does adding SDS in the protein preparation interrupt the use of this antibody? Thanks, Liping
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