MAB377A5
Anti-NeuN Antibody, clone A60, Alexa Fluor™ 555 Conjugate
clone A60, from mouse, ALEXA FLUOR™ 555
Synonym(s):
NEUronal Nuclei, Neuronal Nuclei
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 555
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
A60, monoclonal
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
General description
NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
Application
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected NeuN in mouse adult brain cryosections.
This Anti-NeuN Antibody, clone A60, Alexa Fluor 555 Conjugate is validated for use in ICC, IHC for the detection of NeuN.
Quality
Evaluated by Immunocytochemistry in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 primary cortex cells.
Target description
The unconjugated parent antibody (Cat. No. MAB377) has an observed molecular weight in the 46-48 kDa range and possibly another band at approximately 66 kDa
Other Notes
Unconjugated Anti-NeuN has been shown to work with multiple species including Human, Ferret, Chicken, and Avian.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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