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Trypsin, Porcine, MS Grade

Name: Trypsin, Porcine, MS Grade
Brand: MM

Synonym(s):

Porcine Trypsin

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About This Item

NACRES:

NA.54

UNSPSC Code:

12352202

Specific activity:

≥10,000 U/mg

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Properties

grade

Proteomics Grade

Quality Segment

100

form

lyophilized

specific activity

≥10,000 U/mg

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

suitability

suitable for mass spectrometry

foreign activity

Chymostrypsin <3 BTEE units/mg solid

shipped in

wet ice

storage temp.

−20°C

Description

General description

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.

Preparation Note

Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.

Other Notes

Henzel, W.J., et al. 1993. PNAS90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..

One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.

One BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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This Item	EMS0007	EMS0006	EMS0005
Sigma-Aldrich 650279 Trypsin, Porcine, MS Grade	Sigma-Aldrich EMS0007 Recombinant Trypsin Dimethlyated	Sigma-Aldrich EMS0006 Recombinant Trypsin	Sigma-Aldrich EMS0005 SOLu-Trypsin Dimethylated
description -	description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris	description Proteomics Grade, lyophilized powder, recombinant, expressed in Pichia pastoris	description Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution
specific activity ≥10,000 U/mg	specific activity ≥10,000 units/mg protein	specific activity ≥10,000 units/mg protein	specific activity ≥10,000 units/mg protein (Enzymatic activity)
grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade	grade Proteomics Grade
form lyophilized	form lyophilized powder	form lyophilized powder	form ready-to-use solution
suitability suitable for mass spectrometry	suitability suitable for mass spectrometry	suitability suitable for , suitable for mass spectrometry	suitability suitable for mass spectrometry
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

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