48-660MAG

MILLIPLEX^® MAPK/SAPK Signaling Panel

Premixed MAPK/SAPK Phosphoprotein 10-Plex Cell Signaling Panel

• Synonym(s): Cell Signaling Kit, MAPK/SAPK 10-Plex Kit, MAPK SAPK signaling panel, MAPK phosphorylation detection, SAPK phosphoprotein biomarkers, cell stress signaling panel, rat MAPK pathway assay, stress kinase multiplex assay
• UNSPSC Code: 12161503
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• Product Name: MILLIPLEX^® MAPK/SAPK Signaling Panel,
• Quality Segment: 200
• description: Premixed MAPK/SAPK Phosphoprotein 10-Plex Cell Signaling Panel
• species reactivity: human
• packaging: pkg of 1 ea
• manufacturer/tradename: Milliplex^®
• technique(s): multiplexing: suitable
• input: cell lysate
• assay range: inter-assay cv: 4-13%; intra-assay cv: 3-10%
• detection method: fluorometric (Luminex^® xMAP^® technology)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

This panel focuses on phosphorylated proteins within the MAPK and SAPK pathways, which regulate cellular responses to stress, growth factors, and cytokines. ERK1/2 (pT185/pY187), JNK (pT183/pY185), and p38 (pT180/pY182) are core MAPKs involved in proliferation, apoptosis, and inflammation. MEK1 (pS217/pS221) activates ERK, while MSK1 (pS376) and RSK1 (pS380) are downstream effectors. c-Jun (pS73) and ATF2 (pT71) are transcription factors regulated by JNK and p38. HSP27 (pS82) and MKK3/6 (pS189/pS207) are involved in stress response and MAPK activation.

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 10 analytes in cell lysate samples.

Analytes Included: ERK1/2 (pT185/pY187), JNK (pT183/pY185), p38 (pT180/pY182), MEK1 (pS217/pS221), MSK1 (pS376), RSK1 (pS380), c-Jun (pS73), ATF2 (pT71), HSP27 (pS82), MKK3/6 (pS189/pS207)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate^™).

Features and Benefits

• Simultaneously detect 10 phosphorylated proteins in MAPK and SAPK signaling pathways
• Includes ERK, JNK, p38, MEK1, MSK1, RSK1, c-Jun, ATF2, HSP27, and MKK3/6
• Magnetic bead-based format ensures reproducibility and efficient processing
• Compatible with cell lysates from stress response, apoptosis, and inflammation studies
• Ideal for toxicology, oncology, and cell biology research
• Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

Legal Information

Luminex is a registered trademark of Luminex Corp

MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• hcodes: H302,H315,H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2