Skip to Content
Merck
All Photos(1)

Key Documents

475821

Sigma-Aldrich

Microcystin-LR

from Microcystis aeruginosa, ≥95% (HPLC), liquid, protein phosphatase 2A and protein phosphatase 1 inhibitor, Calbiochem®

Synonym(s):

InSolution Microcystin-LR, Microcystis aeruginosa

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C49H74N10O12
Molecular Weight:
995.17
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Microcystin-LR, Microcystis aeruginosa, InSolution, ≥95%, inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)

Quality Level

Assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

shipped in

wet ice

storage temp.

−20°C

General description

Cyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.

Biochem/physiol Actions

Cell permeable: no
Primary Target
protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A)
Product does not compete with ATP.
Reversible: no
Target IC50: 1.7 nM, 40 pM, against protein phosphatase 1 (PP1), protein phosphatase 2A (PP2A), respectively

Packaging

Packaged under inert gas
yes

Warning

Toxicity: Highly Toxic (H)

Physical form

A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475815) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J.287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol.116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
MacKintosh, C., et al. 1990. FEBS Lett.264, 187.
Cohen, P., et al. 1989. FEBS Lett.250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins3,Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

Not available for sale outside of the United States.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup - (Dimethylsulfoxide)

Flash Point(C)

87 °C - closed cup - (Dimethylsulfoxide)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Alice S Ryan et al.
Obesity (Silver Spring, Md.), 32(4), 667-677 (2024-02-28)
The authors sought to understand sex differences in muscle metabolism in 73 older men and women. Body composition, VO2max, and insulin sensitivity (M) by 3-hour hyperinsulinemic-euglycemic clamp with vastus lateralis muscle biopsies were measured. Women had lower body weight, VO2max
Maria Casas et al.
Nature communications, 14(1), 4553-4553 (2023-07-29)
Lysosomes communicate through cholesterol transfer at endoplasmic reticulum (ER) contact sites. At these sites, the Niemann Pick C1 cholesterol transporter (NPC1) facilitates the removal of cholesterol from lysosomes, which is then transferred to the ER for distribution to other cell

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service