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β-Galactosidase, E. coli

Name: β-Galactosidase, <i>E. coli</i>
Brand: MM

Synonym(s):

β-Galactosidase, E. coli, β-D-Galactoside galactohydrolase

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About This Item

CAS Number:

9031-11-2

EC Number:

3.2.1.23 (BRENDA, IUBMB)

NACRES:

NA.32

UNSPSC Code:

12352202

MDL number:

MFCD12912442

Specific activity:

≥600 units/mg protein

Biological source:

Escherichia coli

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Properties

biological source

Escherichia coli

Quality Segment

200

description

RTECS - LW5840000

form

lyophilized solid

specific activity

≥600 units/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

color

white

solubility

water: 2%

shipped in

ambient

storage temp.

−20°C

Description

General description

Native E. coli β-galactosidase. Inducible enzyme not found in eukaryotic cells that is used as a reporter gene. Used to assess efficiency of transfection. Also used in ELISA and immunocytochemical techniques. Histochemical substrates include X-Gal (Cat. No. 203782).

Packaging

Yes

Other Notes

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of p-nitrophenyl-β-galactoside per min at 37°C.

Zeleny, R., et al. 1997. Anal. Biochem.246, 96.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	G6008	345804	345797
Sigma-Aldrich 345788 β-Galactosidase, E. coli	Sigma-Aldrich G6008 β-Galactosidase from Escherichia coli	Sigma-Aldrich 345804 β1-3,6-Galactosidase, Recombinant, E. coli	Sigma-Aldrich 345797 α1-3,6-Galactosidase, Recombinant, E. coli
description -	description Grade VI, lyophilized powder, ≥250 units/mg protein	description -	description Catalyzes the hydrolysis of α1,3-linked and α1,6-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.
biological source Escherichia coli	biological source Escherichia coli	biological source -	biological source -
specific activity ≥600 units/mg protein	specific activity ≥250 units/mg protein	specific activity ≥30 units/mL, ≥75 units/mg protein	specific activity ≥30 units/mg protein, ≥85 units/mL
form lyophilized solid	form lyophilized powder	form liquid	form liquid
solubility water: 2%	solubility -	solubility -	solubility -
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in wet ice	shipped in wet ice	shipped in wet ice

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

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