Calcein-AM is soluble directly in anhydrous DMSO at 5 mg/mL. However, the stock solution is typically diluted to a range of 0.1-10 µM in buffer or medium for cell staining. It is not recommended to add the DMSO stock solution directly to cells.
206700
Calcein-AM
A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein.
Synonym(s):
Calcein-AM, Calcein-acetoxymethyl Ester, Diacetate, CAL-AM
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About This Item
Empirical Formula (Hill Notation):
C46H46N2O23
CAS Number:
Molecular Weight:
994.86
UNSPSC Code:
12171500
NACRES:
NA.25
MDL number:
Pricing and availability is not currently available.
Quality Segment
assay
≥90% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
off-white
solubility
DMSO: 5 mg/mL
fluorescence
λex 494 nm, λem 517 nm
shipped in
ambient
storage temp.
−20°C
SMILES string
N(Cc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)C42OC(=O)c5c4cccc5)(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
InChI
1S/C46H46N2O23/c1-25(49)60-21-64-41(55)17-47(18-42(56)65-22-61-26(2)50)15-31-11-35-39(13-37(31)68-29(5)53)70-40-14-38(69-30(6)54)32(12-36(40)46(35)34-10-8-7-9-33(34)45(59)71-46)16-48(19-43(57)66-23-62-27(3)51)20-44(58)67-24-63-28(4)52/h7-14H,15-24H2,1-6H3
InChI key
BQRGNLJZBFXNCZ-UHFFFAOYSA-N
General description
A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. Can also be used for imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, or BCECF, and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis, and multidrug resistance (MDR).
A cell-permeable, nonfluorescent, and hydrophobic compound, which is rapidly hydrolyzed by cytoplasmic esterases inside the cell, releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein (excitation maximum: 494 nm; emission maximum: 517 nm). A cytosolic fluorophore that can also be used imaging mitochondrial permeability pore transition. Calcein is better retained by viable cells than fluorescein, carboxyfluorescein, and BCECF and its fluorescence is brighter in a variety of mammalian cell types. Useful for assessing adhesion, cell viability, chemotaxis and multidrug resistance (MDR).
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Imaging mitochondrial permeability pore transition
Imaging mitochondrial permeability pore transition
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Other Notes
Dhar, S., et al. 1998. Eur. J. Pharmacol. 346, 315.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.
Essodaigui, M., et al. 1998. Biochemistry 37, 2243.
Lemasters, J.J., et al. 1998. Biochim. Biophys. Acta 1366, 177.
Feller, N., et al. 1995. Br. J. Cancer 72, 543.
Feller, N., et al. 1995. FEBS Lett. 368, 385.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description A cell-permeable, non-fluorescent, and hydrophobic compound, which is rapidly hydrolyzed by intracellular esterases releasing the membrane-impermeable, hydrophilic, and intensely fluorescent calcein. | description The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. | description An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. | description BioReagent, suitable for fluorescence, ≥95.0% (HPLC) |
| color off-white | color - | color red | color - |
| fluorescence λem 517 nm, λex 494 nm | fluorescence λex 480 nm; λem 320 nm | fluorescence λex ~490 nm, λem ~518 nm | fluorescence λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+ |
| solubility DMSO: 5 mg/mL | solubility DMSO: 5 mg/mL | solubility DMSO: 1 mg/mL | solubility DMSO: soluble |
| form solid | form lyophilized solid | form lyophilized solid | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in - |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Good morning, is it possible to use CALCEIN-AM alone diluited in DMSO for cells observation on scaffolds? In that case, would it be enough to diluite the product directly in DMSO at a concentration of 5 mg/mL? Thank you in advance
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¿Condiciones de transporte y almacenamiento?
1 answer-
This product stored at -20°C, but ships at ambient temperature. When there may be short-term exposure (i.e. during shipment) to conditions other than those recommended for long-term storage that will not affect product quality, Sigma-Aldrich will ship at ambient temperature.
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