1.52022

Chromolith^® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

• Synonym(s): Chromolith^® HighResolution RP-18 endcapped 100-4.6 HPLC column
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Chromolith^® HighResolution RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column
• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped; endcapped
• packaging: 1 ea of
• extent of labeling: 15% Carbon loading; 18% Carbon loading
• parameter: 200 bar pressure; 200 bar pressure; 45 °C max. temp.; 50 °C max. temp.
• technique(s): HPLC: suitable; HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High); mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 4.6 mm
• surface area: 250 m²/g × 250 m²/g
• matrix: highly porous; monolithic silica; monolithic silica
• matrix active group: C18 bonding phase, octadecylsilane (ODS); C18 bonding phase
• pore size: 1.15 μm macroporosity; 1.5 μm macroporosity; 15 nm mesoporosity; 15 nm mesoporosity
• operating pH range: 2-7.5; 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith^® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high resolution separations of complex compound mixtures on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith^® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith^® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith^® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

Application

• A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith^® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).

Analysis Note

Appearance: passes test
Pressure: 40 - 70 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 140000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids