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1.15363

Milk Powder for Biotechnology and Microbiology

for Biotechnology and Microbiology

Synonym(s):

Skim milk powder, Dry skim milk, non-fat milk solids, skimmed milk powder,

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About This Item

CAS Number:
999999-99-4
MDL number:
MFCD00148415
UNSPSC Code:
41106212
NACRES:
NA.74
EC Index Number:
310-127-6
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Product Name

Skim milk powder, suitable for microbiology

biological source

bovine milk

Quality Segment

200

form

solid

shelf life

limited shelf life, expiry date on the label

packaging

pkg of 500 g

pH

6-8 (20 °C, 20 g/L in H2O)

solubility

700 g/L

bulk density

500 kg/m3

application(s)

microbiology, pathogen testing

storage temp.

15-25°C

General description

Skim milk powder is widely used in microbiology as a nutrient source in microbial culture media. It provides a complex mixture of nutrients, including protein, lactose, and minerals, that support the growth of a variety of microorganisms. Skim milk powder is commonly used in media formulations for the cultivation of lactic acid bacteria, including Streptococcus and Lactobacillus species. It can also be used as a supplement in media for the growth of other microbial cells, including fungi, yeasts, and some bacteria that utilize milk proteins as a nutrient source. Additionally, skim milk powder has the advantage of being shelf-stable and easily rehydratable, making it a convenient and cost-effective source of nutrients for microbial cultivation.

Application

Skim milk powder is usually used as an ingredient in many microbial culture media for the growth and identification of bacteria, yeasts, and molds from various pharmaceutical, environmental, food and beverage samples. Furthermore, it is described in the EN ISO 6887-4:2017 to be used as pre-enrichment broth for the detection of Salmonella spp. and STEC when testing cocoa and cocoa containing products (use 100 g/l; sterilize after reconstitution). As an ingredient in culture media Skim milk powder is usually employed in concentrations of 0.1 to 1.0%.

Analysis Note

Assay protein (ex N, calc. on dried substance): ≥ 35 %
Lactose (enzymatically): 40 - 52 %
pH-value (1 %; water): 6 - 7
Fats: ≤ 1.5 %
Free acid (as lactic acid): ≤ 2 %
Ash (800 °C): ≤ 10 %
Water (according to Karl Fischer): ≤ 5.0 %
Suitability for microbiology: passes test
Microbiological test: passes test
Identity (NIR): passes test

Other Notes

Rehydrating the Skim milk powder, or using it for the preparation of culture media, the milk powder should be gently added to water with proper stirring to make sure that no clumps are present in the rehydrated skim milk or the prepared media
Replaces: 1.15363.9017

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Skim milk powder suitable for microbiology

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1.15363

Skim milk powder

M-Gredient® Skim Milk Powder for Biotechnology and Microbiology

Millipore

70166

M-Gredient® Skim Milk Powder

Cobalt powder 99+

Sigma-Aldrich

1.12211

Cobalt powder

vibrant-m

BCR188

Spiked milk powder (pesticides)

form

solid

form

powder

form

solid

form

-

application(s)

microbiology, pathogen testing

application(s)

food and beverages
pathogen testing, microbiology

application(s)

-

application(s)

-

biological source

bovine milk

biological source

bovine milk

biological source

-

biological source

-

storage temp.

15-25°C

storage temp.

-

storage temp.

no temp limit

storage temp.

2-8°C

solubility

700 g/L

solubility

-

solubility

-

solubility

-

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

shelf life

-

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  

  1. Can Milk Powder for Biotechnology and Microbiology (Product code-1.15363) be used in ELISA Assays?

    1 answer

    1. Although some ELISAs utilize casein (C7078) or non-fat milk (1153630500), this application has not been tested and is not included in the Certificate of Analysis specifications for these 2 products. There are references in protocols that cite the use of non-fat dry milk for blocking in ELISA, but it is up to the end-user to determine cross-reactivity within their assay.

      We recommend as blocking reagent for ELISA, BSA at 1-5% concentration. The following products can be considered: 126593, A7030, A3059, 810531 or our Probumin BSAs for Diagnostic Kit Manufacturers.

      Helpful?

  2. Can I autoclave the milk solution prepared from this product?

    1 answer

    1. Although not mentioned in the product datasheet, 1153630500 is meant to be used for the preparation of culture media and as such can indeed be autoclaved.

      Helpful?

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