07-574

Anti-CENP-A Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-CENP-A, Anti-CenH3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 15

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_001809
• UniProt accession no.: P49450
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CENPA(1058)

Description

General description

17kDa

CENP-A is a variant version of histone H3 found only at centromeres.

Centromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromation structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.

Immunogen

peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A

Application

Anti-CENP-A Antibody is a Rabbit Polyclonal Antibody for detection of CENP-A also known as Centromere autoantigen A & has been tested in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Broad species cross-reactivity is predicted.

CENP-A

Physical form

Affinity purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

ImmunoAffinity Purified

Preparation Note

Stable for 1 year at 2 to 8ºC from date of receipt.

Analysis Note

Control
Control Histones (from Hela cells) colcemid-treated or untreated

routinely evaluated by immunoblot in acid extracted histones from HeLa cells

Other Notes

Replaces: 04-205

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1