06-868

Anti-GABAA Receptor α1 Antibody

Upstate^®, from rabbit

• Synonym(s): Gamma-amino butyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, gamma-aminobutyric acid (GABA) A receptor, alpha 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: Np_899155
• UniProt accession no.: P62813
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Gabra1(14394) ; rat ... Gabra1(29705)

Description

General description

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

Specificity

Other homologies: Human (94% sequence homology).

This antibody recognizes the topographical domain of GABAA Receptor α1.

Immunogen

Epitope: Topological domain.

KLH-conjugated linear peptide corresponding to the topological domain of rat GABAA Receptor α1.

Application

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABAA Receptor α1 in rat hippocampus tissue.

Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated GABAA Receptor α1 in rat brain microsomal preparation RIPA lysate.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-GABAA Receptor α1 Antibody is validated for use in IH, IP, WB for the detection of GABAA Receptor α1.

Quality

Evaluated by Western Blot in rat brain microsomal preparation tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected GABAA Receptor α1 in 10 µg of rat brain microsomal preparation tissue lysate.

Target description

~51 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat Brain Microsomal Preparation Tissue Lysate.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1