05-175

Anti-Myb Antibody, clone 1-1

clone 1-1, Upstate^®, from mouse

• Synonym(s): Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA), v-myb avian myeloblastosis viral oncogene homolog, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral on
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1-1, monoclonal
• Application: IHC, IP, WB
• Citations: 78

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1-1, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NM_005375.2
• UniProt accession no.: P10242
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MYB(4602)

Description

General description

80 kDa

Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5′-YAAC[GT]G-3′. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Immunogen

Recombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.

Application

Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.

Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.

Immunohistochemistry:

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Biochem/physiol Actions

This antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).

Physical form

Ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography.

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.

Preparation Note

Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Routinely evaluated on RIPA lysate from Jurkat cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable