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927554

MI-alkyne

≥95%

Synonym(s):

N-(2-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)hex-5-ynamide

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About This Item

Empirical Formula (Hill Notation):
C12H14N2O3
Molecular Weight:
234.25
NACRES:
NA.21
UNSPSC Code:
12352101
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Quality Segment

assay

≥95%

storage temp.

−20°C

Application

MI-alkyne is a Michael acceptor probe that can be used to lable cysteines. A method was developed using cysteine-reactive compounds including this one to allow for unbiased analysis of proteomic data in quantitave applications (Zanon et al. 2021). The method uses light or heavy labeling with the isotopically labelled desthiobiotin azide (isoDTB) tag for mass spectrometry analysis (Zanon et al. 2020). Analysis then uses the isotopic tandem orthogonal proteolysis activity-based protein profiling (isoTOP-ABPP) workflow (Weerapana et al. 2010, Backus et al. 2016).

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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