219215

Leucocrystal Violet

• Synonym(s): 4,4′,4′′-Methylidynetris(N,N-dimethylaniline), Leuco Crystal Violet
• Linear Formula: CH[C₆H₄N(CH₃)₂]₃
• CAS Number: 603-48-5
• Molecular Weight: 373.53
• NACRES: NA.47
• PubChem Substance ID: 24853114
• UNSPSC Code: 12171500
• EC Number: 210-043-9
• MDL number: MFCD00008314

Properties

• form: powder
• Quality Segment: 200
• technique(s): microbe id | staining: suitable
• mp: 175-177 °C (lit.)
• λmax: 260 nm
• ε (extinction coefficient): ≥700 at 257-263 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CN(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C
• InChI: 1S/C25H31N3/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6/h7-18,25H,1-6H3
• InChI key: OAZWDJGLIYNYMU-UHFFFAOYSA-N

Description

Application

Leucocrystal Violet has been used to identify histamine-producing lactobacilli in cheese.

Biochem/physiol Actions

Leucocrystal Violet (LCV) is a cationic triarylmethane dye. LCV is a reduced form of gentian violet (crystal violet) and thus, it is colorless. It has an affinity for both cellulosic and proteinaceous materials. LCV and hydrogen peroxide react with the hemoglobin in blood and turn the blood impression to purple/violet color. Therefore, the dye is widely used to stain blood residue on both porous and non-porous materials.

Disclaimer

Photo-ionization can cause color variations

Safety Information

• pictograms: GHS09
• signalword: Warning
• hcodes: H410
• pcodes: P273 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves