215430

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone

99%

• Synonym(s): N-(4-Pyridylmethylene)-tert-butylamine N,N′-dioxide, N-tert-Butyl-α-(4-pyridyl)nitrone N′-oxide, POBN
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂O₂
• CAS Number: 66893-81-0
• Molecular Weight: 194.23
• UNSPSC Code: 12352100
• NACRES: NA.22
• PubChem Substance ID: 24852894
• EC Number: 266-512-3
• Beilstein/REAXYS Number: 1453764
• MDL number: MFCD00041022
• Assay: 99%
• Form: solid

Properties

• Quality Segment: 200
• assay: 99%
• form: solid
• mp: 183-185 °C (lit.)
• solubility: water: soluble 10 mg/mL, clear, colorless to faintly yellow
• functional group: amine
• SMILES string: CC(C)(C)\[N+]([O-])=C/c1cc[n+]([O-])cc1
• InChI: 1S/C10H14N2O2/c1-10(2,3)12(14)8-9-4-6-11(13)7-5-9/h4-8H,1-3H3/b12-8+
• InChI key: RNRMWTCECDHNQU-XYOKQWHBSA-N

Description

Application

α-(4-Pyridyl N-oxide)-N-tert-butylnitrone was used as spin trapping agent to detect:

• the formation of hydroxyl radical adducts during decomposition of hydrogen peroxide in the presence of nickel (II) oligopeptides
• hydroxyl radicals and selenide radical anions

Spin trap reagent used to measure ethanol oxidation to 1-hydroxyethyl radical (HER) which can bind to protein. Has also been used in studies of DNA methylation, iron-mediated polyunsaturated fatty acid peroxidation, and free-radical formation by Alzheimer β-amyloid peptide. The formation of hydroxyl radical in chondrocytes and cartilage has been detected using POBN.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)