G005745
HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC
application for HPLC
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About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] water; [B] acetonitrile; (25:75, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL
suitability
application for HPLC
application(s)
food and beverages
Analysis Note
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
analytical column
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