T3253

Trizma^® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

• Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• EC Number: 214-684-5
• MDL number: MFCD00012590
• UNSPSC Code: 12161700
• PubChem Substance ID: 24900119
• NACRES: NA.25

Properties

• grade: reagent grade
• Quality Level: 400
• Assay: ≥99.0% (titration)
• form: crystalline
• storage condition: dry at room temperature
• technique(s): affinity chromatography: suitable
• impurities: ≤0.5% water (Karl Fischer)
• color: white
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• mp: 150-152 °C
• solubility: H₂O: soluble, clear, colorless
• density: 1.39 g/cm³ at 20—25 °C; 1.41 g/cm³ at 20—25 °C
• absorption: ≤0.05 at 290 nm at 40%
• suitability: suitable for mass spectrometry
• application(s): diagnostic assay manufacturing; life science and biopharma; sample preparation
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Tris HCl, or Trizma^® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9.

Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.

Application

Trizma^® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

Features and Benefits

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7-9 with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

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The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves