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Merck

SAB4200750

Sigma-Aldrich

Anti-Gelsolin antibody, Mouse Monoclonal

clone GS-2C4, purified from hybridoma cell culture

Synonym(s):

Anit-AGEL, Anit-Actin-depolymerizing factor (ADF), Anit-GSN

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GS-2C4, monoclonal

form

buffered aqueous solution

mol wt

~85 kDa

species reactivity

rabbit, canine, human, mouse, monkey

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1-2.5 μg/mL using MDCK cell line extract
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections
immunoprecipitation (IP): suitable

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This Item
G4896WH0002934M1SAB4200710
antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

GS-2C4, monoclonal

clone

GS-2C4, monoclonal

clone

3G5, monoclonal

clone

hCP, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

-

species reactivity

rabbit, canine, human, mouse, monkey

species reactivity

bovine, pig, human, rabbit

species reactivity

human

species reactivity

bovine, monkey, human, rabbit, ferret, mouse, hamster, rat, canine, pig

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma).

Immunogen

Human plasma gelsolin

Application

Anti-Gelsolin antibody, Mouse Monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Topological assignment of the N-terminal extension of plasma gelsolin to the gelsolin surface
Ulrike F, et al.
The Biochemical Journal, 385(3), 659-665 (2005)
Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study
Megger DA, et al.
Molecular and Cellular Proteomics, 12(7), 2006-2020 (2013)
Gelsolin amyloidosis: genetics, biochemistry, pathology and possible strategies for therapeutic intervention
Solomon JP, et al.
Critical Reviews in Biochemistry and Molecular Biology, 47(3), 282-296 (2012)
Vesicular egress of non-enveloped lytic parvoviruses depends on gelsolin functioning
Bar S, et al.
PLoS Pathogens, 4(8), e1000126-e1000126 (2008)
Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4, 5-bisphosphate-dependent organization of cell actin
Kwik J, et al.
Proceedings of the National Academy of Sciences of the USA, 100(24), 13964-13969 (2003)

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