S5947

Anti-Sirt7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-SIR2L7, Anti-Sir2-related protein type 7, Anti-Sirtuin (silent mating type information regulation 2 homolog) 7
• MDL number: MFCD07784768
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~45 kDa
• species reactivity: mouse (predicted), human
• technique(s): immunoprecipitation (IP): 2-4 μg using extracts of HEK-293T cells expressing human Sirt7; indirect immunofluorescence: 2-4 μg/mL using human HEK-293T cells; western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing human Sirt7
• UniProt accession no.: Q9NRC8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SIRT7(51547) ; mouse ... Sirt7(209011)

Description

General description

Nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase (Sir2) is one of the silent information regulator genes in yeast. It belongs to a family of proteins, that is found in organisms ranging from bacteria to complex eukaryotes. Proteins of this family share a core domain showing 25-60% sequence identity. The mammalian Sir2 gene family is comprised of seven members which are designated as Sirt1-7.

Immunogen

synthetic peptide corresponding to amino acids 35-51 of human Sirt7, conjugated to KLH via a C-terminal cysteine residue. The sequence is identical in mouse.

Application

Anti-Sirt7 antibody produced in rabbit has been used in western blotting analysis and immunofluorescence detection.

Biochem/physiol Actions

Sirt7 (sirtuin 7) is extensively found in protein associated with active rRNA genes (rDNA), in the nucleolus. It interacts with RNA polymerase I (Pol I) and histones. Overexpression of Sirt7 increases Pol I-mediated transcription, whereas knockdown of Sirt7 or inhibition of its catalytic activity results in decreased association of Pol I with rDNA and reduces Pol I transcription. Depletion of Sirt7 stops cell proliferation and triggers apoptosis. High levels of Sirt7 expression are associated with breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable