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S5188

Sigma-Aldrich

Anti-SGK antibody produced in rabbit

buffered aqueous solution, IgG fraction of antiserum

Synonym(s):

Anti-SGK

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About This Item

MDL number:
MFCD02686615
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human

technique(s)

indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line
microarray: suitable
western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line

UniProt accession no.

O00141

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SGK1(6446)

General description

Anti-SGK is developed in rabbit using a synthetic peptide corresponding to the C-terminus of human SGK conjugated to KLH (keyhole limpet hemocyanin). SGK is localized to the perinuclear or cytoplasmic compartment as a hypophosphorylated protein.
SGK (serum and glucocorticoid-regulated protein kinase) is a 50 kDa member of Ser/Thr kinase family. Sequence of sgk is well conserved across species, the human and rat SGK amino acid sequences are 96-98% identical.

Application

Anti-SGK antibody produced in rabbit has been used in :
  • immunoprecipitation
  • western blotting
  • immunofluorescence

Biochem/physiol Actions

SGK (serum and glucocorticoid-regulated protein kinase) plays an important role in signal transduction activated by mineral corticoids. It is also regulated at the transcription level by stimuli including glucocorticoid, serum, follicle stimulating hormone as well as changes in cell volume. SGK levels are strongly affected by osmotic changes. SGK is strongly and rapidly stimulated in kidney cells in response to aldosterone and stimulates epithelial Na+ channels, suggesting a central role of SGK in the regulation of sodium transport and homeostasis. SGK transcription is markedly increased in diabetic nephropathy, in response to excessive extracellular glucose concentrations. The localization of SGK in the cell is regulated by hormones or serum. For cell cycle progression, the shuttling of SGK between nucleus and cytoplasm is required.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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A Náray-Fejes-Tóth et al.
Kidney international, 57(4), 1290-1294 (2000-04-12)
The sgk, an aldosterone-induced gene in mineralocorticoid target cells, regulates the epithelial sodium channel. Aldosterone increases sodium reabsorption in tight epithelia. The early phase of this stimulatory effect is thought to involve activation of apical sodium channels. To identify immediate-early
cell cycle and hormonal control of nuclear-cytoplasmic localization of the serum-and glucocorticoid-inducible protein kinase, sgk, in mammary tumor cells a novel convergence point of anti-proliferative and proliferative cell signaling pathways
Buse P, et al.
The Journal of biological chemistry, 274(11), 7253-7263 (1999)
Joseph R Shaw et al.
Aquatic toxicology (Amsterdam, Netherlands), 98(2), 157-164 (2010-03-09)
Seawater acclimation in killifish, Fundulus heteroclitus, is mediated in part by a rapid (1h) translocation of CFTR Cl(-) channels from an intracellular pool to the plasma membrane in gill and increased CFTR-mediated Cl(-) secretion. This effect is mediated by serum
Sgk is an aldosterone-induced kinase in the renal collecting duct Effects on epithelial Na+ channels
Naray FTA, et al.
The Journal of Biological Chemistry, 274(24), 16973-16978 (1999)
K v7 channels in the nucleus accumbens are altered by chronic drinking and are targets for reducing alcohol consumption
McGuier NS, et al.
Addiction Biology, 21(6), 1097-1112 (2016)
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