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Key Documents

P8997

Sigma-Aldrich

Anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-P2Z

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human, rat, mouse

technique(s)

flow cytometry: 10 μg/test using Jurkat T-cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... P2RX7(5027)
mouse ... P2rx7(18439)
rat ... P2rx7(29665)

Related Categories

General description

Purinergic Receptor P2X7 is a ionotrophic ATP-gated channel responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. ATP activation of the P2RX7 leads to recruitment of pannexin pores which allow small molecules such as ATP to leak out of cells further activating of purinergic receptors and physiological responses such a spreading cytoplasmic waves of calcium.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.

Specificity

Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody reacts with rat, human, and mouse purinergic receptor P2X7 (extracellular).

Immunogen

synthetic peptide corresponding to residues 136-152 of mouse P2X7 receptor. Sequence homology with human, mouse, and rat is identical. Bovine is 14/17 residues identical.

Application

Rabbit polyclonal anti-Purinergic Receptor P2X7 (extracellular)−FITC antibody is used to tag Purinergic Receptor P2X7 (extracellular) for detection and quantitation by fluorescence-based immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Purinergic Receptor P2X7 (extracellular) in ATP-dependent processes such as cell lysis and calcium movement.

Physical form

Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jasmine M Manouchehri et al.
Frontiers in oncology, 12, 855032-855032 (2022-05-07)
Breast cancer affects two million patients worldwide every year and is the most common cause of cancer-related death among women. The triple-negative breast cancer (TNBC) sub-type is associated with an especially poor prognosis because currently available therapies fail to induce
Mingxuan Li et al.
Journal of immunology (Baltimore, Md. : 1950), 205(7), 1752-1762 (2020-09-02)
P2X7 receptor (P2X7R) is highly expressed on immune cells, triggering the release of cytokines and regulating autoimmune responses. To investigate P2X7R surface expression on T helper (Th) 1, Th17, and regulatory T (Treg) cells in patients with systemic lupus erythematosus

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