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L6261

Sigma-Aldrich

Lipopolysaccharides from Salmonella enterica serotype minnesota

purified by phenol extraction

Synonym(s):

LPS

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About This Item

MDL number:
MFCD00131520
UNSPSC Code:
12352204
NACRES:
NA.83

biological source

Salmonella enterica (serotype minnesota)

Quality Level

200

form

lyophilized powder

purified by

phenol extraction

impurities

<3% Protein (Lowry)

solubility

water: soluble

storage temp.

2-8°C

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General description

Lipopolysaccharide (LPS) is the pyrogenic element arising from enterobacteria such as Escherichia coli and Salmonella enterica. It is an amphiphilic component containing polysaccharides and lipoidic material. LPS is present in the outer membrane of the cell wall of almost all Gram-negative bacteria.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Application

Lipopolysaccharides from Salmonella enterica serotype minnesota has been used:
  • as a toll-like receptors (TLR ) agonist to induce tumor necrosis factor (TNF) α production in cereblon and Rabex-5 deficient mice in vivo,
  • to study its effect on prostaglandin-E Synthase (PTGES) protein expression in monocyte-derived macrophages
  • as a positive control for the maturation of monocyte-derived dendritic cells (MoDCs) to assess secretion of interleukin-10 and interferon (IFN)-γ
  • as a supplement in Roswell park memorial institute (RPMI)media for culturing splenic B cells
  • as a toll-like receptor 4 ligand to study its effect on the expression of the proallergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL33) in H292 cells (pulmonary mucoepidermoid carcinoma cells)

Biochem/physiol Actions

Lipopolysaccharide (LPS) forms a permeability barrier around the cell surface to restrict the entry of toxic molecules such as antibiotics and bile salts. Owing to their external location, this endotoxin also plays a vital role in host-bacterium interactions by regulating host immune response. LPS is a microbe-associated molecular pattern (MAMP) that serves as a potent agonist for toll-like receptor 4 (TLR4)-MD2-CD14 receptor complex.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Mohammed A Khedr et al.
European journal of gastroenterology & hepatology, 27(10), 1170-1174 (2015-07-07)
Hepatitis C virus (HCV) infection is a major health problem worldwide. Defective dendritic cell (DC) activation of T cells may underlie poor T-cell responsiveness in HCV infection. To evaluate the DCs' functions in chronically infected HCV patients and its correlation
David Millrine et al.
International immunology, 28(6), 307-315 (2016-02-13)
Thalidomide and its derivatives, collectively referred to as immunomodulatory drugs (IMiDs), are effective inhibitors of inflammation and are known to inhibit TLR-induced TNFα production. The identification of Cereblon as the receptor for these compounds has led to a rapid advancement
Shanye Yin et al.
Cancer cell, 35(2), 283-296 (2019-02-05)
SF3B1 is recurrently mutated in chronic lymphocytic leukemia (CLL), but its role in the pathogenesis of CLL remains elusive. Here, we show that conditional expression of Sf3b1-K700E mutation in mouse B cells disrupts pre-mRNA splicing, alters cell development, and induces
September R Mihaly et al.
PloS one, 9(4), e94982-e94982 (2014-04-17)
Macrophages play diverse roles in tissue homeostasis and immunity, and canonically activated macrophages are critically associated with acute inflammatory responses. It is known that activated macrophages undergo cell death after transient activation in some settings, and the viability of macrophages
Srinivas Mamidi et al.
Immunobiology, 217(6), 593-600 (2011-12-30)
All-trans retinoic acid (ATRA) is a potent retinoid, which has been used successfully in different clinical settings as a potential drug to treat COPD and emphysema. In the present study, we analyzed genes modulated by ATRA by performing mRNA expression
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Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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