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L4402

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

Synonym(s):

Glycyl-glycine Endopeptidase

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About This Item

CAS Number:
9011-93-2
Enzyme Commission number:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Staphylococcus sp.)

Quality Level

300

product line

BioUltra

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

specific activity

≥2,000 units/mg protein

mol wt

25 kDa

composition

Protein, 40-60% biuret

concentration

40—60% protein

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

antibiotic activity spectrum

Gram-positive bacteria

application(s)

diagnostic assay manufacturing

Mode of action

cell wall synthesis | interferes

storage temp.

−20°C

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General description

Chemical structure: peptide
Package size based on protein content

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Preparation Note

Affinity purified

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000202408
0000174306
0000193959
0000202405
0000202381

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Igor Belyansky et al.
The American surgeon, 77(8), 1025-1031 (2011-09-29)
Mesh and wound infections during hernia repair are predominantly caused by Staphylococcus aureus. Human acellular dermis (HAD) is known to lose its integrity in the face of large bacterial loads. The goal of this study was to determine if lysostaphin
Yuliya Yurko et al.
Surgical innovation, 19(1), 20-26 (2011-07-12)
Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh. Lysozyme, human
Hong Wu et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(11), 1623-1630 (2012-03-08)
The purpose of this paper is to establish sulfhydryl site-directed PEGylation method for lysostaphin and to evaluate effects of mutagenesis and modification of amino acid residue within putative linker on enzyme activity. On the basis of structural analysis of lysostaphin
Huanli Liu et al.
Applied and environmental microbiology, 77(24), 8500-8508 (2011-10-11)
Lactobacillus species are a predominant member of the vaginal microflora and are critical in maintaining an acidic vaginal environment thought to contribute to the prevention of a number of urogenital diseases. However, during menstruation the pH of the vaginal environment
Mohammad Mehdi Feizabadi et al.
Journal of microbiological methods, 84(1), 144-146 (2010-11-17)
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as
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