H2519

Heparinase I from Flavobacterium heparinum

Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)

• Synonym(s): Heparin lyase I, Heparinase
• CAS Number: 9025-39-2
• Enzyme Commission number: 4.2.2.7 (BRENDA, IUBMB)
• MDL number: MFCD00131317
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• conjugate: conjugate (Glucosaminoglycan)
• Quality Level: 200
• form: lyophilized solid
• specific activity: ≥200 units/mg protein (enzyme + BSA)
• mol wt: 42.8 kDa
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

Application

Heparinase I from Flavobacterium heparinum has been used:

• in the digestion of plasma samples
• to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
• in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells

Biochem/physiol Actions

Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.

Unit Definition

One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves