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39441

Millipore

Nitrate Reagent B

suitable for microbiology

Synonym(s):

Sulfanilic acid solution, 4-Aminobenzenesulfonic acid

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
41171621
PubChem Substance ID:
NACRES:
NA.85

shelf life

limited shelf life, expiry date on the label

Quality Level

composition

acetic acid (30%), 1000 mL
sulfanilic acid, 8.0 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical

microbiology

suitability

Enterobacter spp.
Escherichia coli
Neisseria spp.
anaerobic bacteria
coliforms

SMILES string

Nc1ccc(cc1)S(O)(=O)=O

InChI

1S/C6H7NO3S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H,8,9,10)

InChI key

HVBSAKJJOYLTQU-UHFFFAOYSA-N

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Application

α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Yan-Qing Wang et al.
Huan jing ke xue= Huanjing kexue, 30(4), 1193-1198 (2009-06-24)
A 4-aminobenzenesulphonate-degrading strain W1 was isolated from the activated sludge of Dalian municipal wastewater treatment plant. Strain W1 was able to utilize 4-ABS as sole carbon and energy source under aerobic condition. It was identified as Pannonibacter sp. according to
Kwan Kim et al.
The journal of physical chemistry. B, 109(40), 18929-18934 (2006-07-21)
Raman scattering measurements were conducted for 4-aminobenzenethiol (4-ABT) assembled on powdered copper substrates. Initially, very weak Raman peaks were detected, but upon attaching Ag nanoparticles probably via NH2 groups onto 4-ABT/Cu, distinct Raman spectra were observed. Considering the fact that
Brian D Piorek et al.
Proceedings of the National Academy of Sciences of the United States of America, 104(48), 18898-18901 (2007-11-21)
We present a microfluidic technique for sensitive, real-time, optimized detection of airborne water-soluble molecules by surface-enhanced Raman spectroscopy (SERS). The method is based on a free-surface fluidic device in which a pressure-driven liquid microchannel flow is constrained by surface tension.
Nam Hoon Kim et al.
Nano letters, 10(10), 4181-4185 (2010-09-25)
A bifunctional adenosine-sensitive double-stranded DNA aptamer was used to create and control a surface-enhanced Raman spectroscopy (SERS) hot spot between a bulk Au surface and a gold nanoparticle (Au NP) attached to the aptamer via a biotin-avidin linkage. The Au
Nam-Jung Kim et al.
Chemical communications (Cambridge, England), (41)(41), 6246-6248 (2009-10-15)
An elusive chemical contribution to SERS is investigated by estimating the chemical coupling strengths through a dynamic relative peak analysis during solvent evaporation from a diluted chemical sample that is drop-deposited on a soft SERS substrate.

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