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MAB3418A5

Sigma-Aldrich

Anti-MAP2 Antibody, clone AP20, Alexa Fluor™ 555 Conjugate

clone AP20, from mouse, ALEXA FLUOR™ 555

Synonym(s):

Microtubule-associated protein 2, MAP-2, MAP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 555

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

AP20, monoclonal

species reactivity

rat, Xenopus, bovine, chicken, human, quail, mouse

technique(s)

immunocytochemistry: suitable

isotype

IgG

NCBI accession no.

NM_001205807

UniProt accession no.

F1MEW3

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

chicken ... Map2(424001)
frog ... Map2(780043)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)

General description

The microtubule-associated protein 2 (MAP-2) is an abundant neuronal cytoskeletal protein that binds to tubulin, stabilizes microtubules (Herzog and Weber, 1978), and is an excellent marker for neurons. MAP-2 is essential for the development and maintenance of neuronal morphology (Matus, 1991). Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene), have been identified in neurons. (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP-2 together with MAP-4 and tau proteins belong to the family of thermostable proteins associated with microtubules.

Application

This Anti-MAP2 Antibody, clone AP20, Alexa Fluor 555 Conjugate is validated for use in ICC for the detection of MAP2.

Quality

Evaluated by Immunocytochemistry in rat E18 primary cortex cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MAP2 in rat E18 primary cortex cells.

Target description

~230 kDa observed.

Physical form

Purified mouse monoclonal IgG conjugated to Alexa Fluor™ 555 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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