biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
mol wt
calculated mol wt 550.85 kDa
observed mol wt ~60 kDa
purified by
using protein G
species reactivity
human
species reactivity (predicted by homology)
mouse
packaging
antibody small pack of 100
technique(s)
electron microscopy: suitable
western blot: suitable
isotype
IgGκ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
storage temp.
-10 to -25°C
Gene Information
human ... MUC2(4583)
Specificity
This rabbit polyclonal antibody detects D3 domain of Mucin-2. It targets an epitope within D3 domain from the N-terminal half.
Immunogen
His-tagged recombinant fragment corresponding to 402 amino acids from the D3 domain of human Mucin-2.
Application
Quality Control Testing
Evaluated by Western Blotting with Human Muc2-D3 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
Tested Applications
Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).
Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with Human Muc2-D3 protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Human Muc2-D3 protein.
Tested Applications
Electron Microscopy: A representative lot detected Human Mucin-2 protein in Electron Microscopy applications (Javitt, G., et. al. (2020). Cell.183(3): 717-729).
Western Blotting Analysis: A representative lot detected Human Mucin-2 protein in Western Blotting applications (Javitt, G., et. al. (2020). Cell. 183(3): 717-729).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Mucin-2 (UniProt: Q02817; also known as MUC-2, Intestinal Mucin-2) is encoded by the MUC2 (also known as SMUC) gene (Gene ID: 4583) in human. Mucin-2 (MUC2) is a secreted protein that coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. It provides a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. Mucin-2 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature form. It exists as a disulfide-linked homotrimer that can be highly O-glycosylated. It contains four VWFD domains and two VWFC domains. Mucin-2 undergoes proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer that allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria. The numerous O-glycans on the Mucin-2 are reported to serve as nutrients for the bacteria, serve as attachment sites, and probably contribute to the selection of the species-specific colon flora. Reduced levels of Mucin-2 have been linked to the early stage of colorectal cancer development, which is caused by methylation of the MUC2 promoter. This polyclonal antibody detects D3 domain of mucin-2, which is localized in amino acids 858-1259. (Ref.: Wi, D-H., et al. (2021). BMB Rep. 54(7); 344-355; Javitt, G., et al. (2020). Cell. 183(3); 717-729; Javitt, G., et al. (2019). J. Mol. Biol. 431(19); 3740-3752; Johansson, MEV., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(Suppl. 1); 4659-4665).
Physical form
Purified rabbit polyclonal antibody in PBS without azide.
Reconstitution
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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