07-212

Anti-dimethyl-Histone H3 (Lys9) Antibody

Upstate^®, from rabbit

• Synonym(s): H3K9me2, Histone H3 (di methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: Saccharomyces cerevisiae, human, vertebrates, chicken
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: dry ice
• target post-translational modification: dimethylation (Lys9)
• Gene Information: human ... H3C1(8350)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Expected to cross react with fission yeast and chicken.

Methylated Histone H3 (Lys9). Specificity demonstrated by peptide inhibition assay using methylated and unmethylated peptides. Slight cross-reactivity to the trimethyl Lys27 Histone H3.

Immunogen

Epitope: a.a. 6-13

KLH-conjugated synthetic peptide corresponding to amino acids 6-13 (TAR[dimethyl-K]STGG-C) of Histone H3 with C added to the Cterminus for conjugation. The immunizing sequence is conserved from Tetrahymena to human.

Application

Anti-dimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9) & has been validated in ICC & WB.

Not recommended for ChIP.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3
(cat# 14-411).

Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.

Target description

17 kDa

Physical form

Immunoaffinity Chromatography

Purified rabbit polyclonal IgG in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 5 mg/mL BSA, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
HeLa, NIH/3T3 or COS cell extracts.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable