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  • Organization and dynamics of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: a fluorescence approach.

Organization and dynamics of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-labeled lipids: a fluorescence approach.

Chemistry and physics of lipids (2004-01-07)
Soumi Mukherjee, H Raghuraman, Sudeshna Dasgupta, Amitabha Chattopadhyay
ABSTRACT

Lipids that are labeled with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) group are widely used as fluorescent analogues of native lipids in biological and model membranes to monitor a variety of processes. NBD-labeled lipids have previously been used to monitor the organization and dynamics of molecular assemblies such as membranes, micelles and reverse micelles utilizing the wavelength-selective fluorescence approach. In this paper, we have characterized the organization and dynamics of various NBD-labeled lipids using red edge excitation shift (REES) and other fluorescence approaches which include analysis of membrane penetration depths of the NBD group using the parallax method. We show here that the environment and location experienced by the NBD group of the NBD-labeled lipids could depend on the ionization state of the lipid. This could have potentially important implications in future studies involving NBD-labeled lipids as tracers in a cellular context.

MATERIALS
Product Number
Brand
Product Description

Avanti
18:1-12:0 NBD PS, Avanti Research - A Croda Brand 810195C
Avanti
18:1-12:0 NBD PE, Avanti Research - A Croda Brand 810156P, powder
Avanti
18:1-12:0 NBD PS, Avanti Research - A Croda Brand 810195P, powder
Avanti
18:1-06:0 NBD PE, Avanti Research - A Croda Brand 810155C
Avanti
16:0-12:0 NBD PS, Avanti Research - A Croda Brand 810193C
Avanti
16:0-06:0 NBD PS, Avanti Research - A Croda Brand 810192P, powder
Avanti
16:0-06:0 NBD PS, Avanti Research - A Croda Brand 810192C
Avanti
18:1-06:0 NBD PS, Avanti Research - A Croda Brand