U211200
DMT-2′Fluoro-dU Phosphoramidite
≥99.0% (reversed phase HPLC)
Synonym(s):
DMT-2′-Fluoro-dU amidite
About This Item
biological source
non-animal source (no BSE/TSE risk)
Quality Level
product line
Proligo Reagents
Assay
≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)
form
powder
mol wt
748.78 g/mol
technique(s)
oligo synthesis: suitable
impurities
≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (reversed phase HPLC)
≤3% residual Solvent content
color
white to off-white
λ
conforms (UV/VIS Identity)
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
nucleoside profile
base: deoxyuridine
base protecting group: none
2' protecting group: fluoro
5' protecting group: DMT
deprotection: fast/standard
storage temp.
−20°C
SMILES string
COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
InChI
1S/C39H46FN4O8P/c1-26(2)44(27(3)4)53(50-24-10-22-41)52-36-33(51-37(35(36)40)43-23-21-34(45)42-38(43)46)25-49-39(28-11-8-7-9-12-28,29-13-17-31(47-5)18-14-29)30-15-19-32(48-6)20-16-30/h7-9,11-21,23,26-27,33,35-37H,10,24-25H2,1-6H3,(H,42,45,46)/t33-,35-,36-,37-,53?/m1/s1
InChI key
HQHQPAYRJJMYQX-DJTPZYMWSA-N
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General description
Its key features are-
- Can be employed together with DNA or RNA phosphoramidites
- Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
- Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
- Consistent lot-to-lot high purity and performance
- Manufactured under a certified ISO 9001 quality system
Application
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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