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Key Documents

SAE0071

Sigma-Aldrich

HIV-1 GP120 protein

recombinant, expressed in HEK 293 cells

Synonym(s):

Glycoprotein 120 (gp120), Surface protein gp120

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in HEK 293 cells

Quality Level

description

low endotoxin

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

impurities

≤1 EU/μg protein endotoxin

UniProt accession no.

storage temp.

−20°C

General description

Recombinant human glycoprotein 120 (gp120) is expressed in human HEK 293 cells as an C-terminally his-tagged glycoprotein with a calculated molecular mass of 55 kDa (amino acids Lys33-Arg511, with a C-terminal 8-His tag). The DTT-reduced protein migrates as a 100-130 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding.

Biochem/physiol Actions

Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. It was discovered by Tun-Hou Lee and Myron Essex of the Harvard School of Public Health in 1988. The protein′s name is derived from its apparent molecular weight of 120 kDa. The gp120 protein is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and the CD4 receptor. The presence of gp120 is associated with higher levels of plasma IL-6, IL-10, and TNF-α, which may contribute to immune dysfunction during early HIV infection.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Lichao Peng et al.
Frontiers in neuroscience, 15, 663962-663962 (2021-07-31)
Human immunodeficiency virus envelope glycoprotein 120 (gp120) leads to hyperalgesia. Long non-coding RNAs are characterized by the lack of a protein-coding sequence and may contribute to the development and maintenance of inflammatory and neuroinflammatory pain. Rats with neuroinflammatory pain were

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