SAB4301339

Anti-phospho-4E-BP1 (pThr⁷⁰) antibody produced in rabbit

affinity isolated antibody

• Synonym(s): 4EBP1, P/OKCL.6, PHAS-I
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: 12 kDa
• species reactivity: human
• concentration: 1.0 mg/mL
• technique(s): immunohistochemistry: 1:50-1:100
• isotype: IgG
• accession no.: NP_004086.1.
• UniProt accession no.: Q13541
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pThr70)
• Gene Information: human ... EIF4EBP1(1978)

Description

General description

The gene EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is localized on human chromosome 8p12. It is a translation repressor that interacts with eukaryotic translation initiation factor 4E (eIF4E).

Specificity

The antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70.

Immunogen

Peptide sequence around phosphorylation site of threonine70 (T-K-T(p)-P-P) derived from Human 4E-BP1.

Biochem/physiol Actions

EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) associates with eukaryotic translation initiation factor 4E (eIF4E), which is a multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNA. The interaction of the encoded binding protein with this complex inhibits translation. The phosphorylation of EIF4EBP1 in response to stimuli such as, UV irradiation and insulin signaling, results in dissociation of this factor from the eIF4E complex, leading to initiation of translation of mRNA. This protein participates in cell proliferation, apoptosis, invasion and metastasis. EIF4EBP1 functions as an effector molecule in mTOR (mammalian target of rapamycin complex 1) signaling pathway that regulates protein synthesis. It is found to be overexpressed in hepatocellular carcinoma tissues and serves as a potential prognostic and therapeutic target.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable