F9291

ANTI-FLAG^® BioM2 antibody, Mouse monoclonal

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: M2, monoclonal
• Application: DB
• Citations: 128

Properties

• Quality Segment: 200
• biological source: mouse
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: all
• concentration: ~1 mg/mL
• technique(s): dot blot: suitable (chemiluminescent detection)
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The monoclonal Anti-FLAG BioM2 mouse antibody is covalently attached to biotin by hydrazide linkage. The antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus or C-terminus of FLAG fusion porteins.

Application

Biotin-labeled antibody is used for immunodetection methods using avidin- or streptavidin-conjugated reporter enzymes such as streptavidin-peroxidase. Primary antibody conjugates are preferred when using murine cells as the recombinant protein host.
Antibody is suitable for immunofluorescence, western blotting, microscopy applications and for the formation of avidin-biotin complexes.

Learn more product details in our FLAG^® application portal.

Physical form

Solution in 50% glycerol, 10 mM sodium phosphate, pH 7.25, containing 150 mM NaCl and 0.02% sodium azide

Preparation Note

Dilute antibody in TBS (.05M Tris, pH7.4, with .15M NaCl) to a final concentration of 1-10μg/mL.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable