Unfortunately, this product is not tested for the specific application. A literature search is recommended for further information.
D8515
Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA
lyophilized powder
Synonym(s):
DNA, Deoxyribonucleic acid, ds-DNA-Cellulose, DNA-Cellulose
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About This Item
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
Pricing and availability is not currently available.
biological source
bovine (calf) thymus
Quality Segment
form
lyophilized powder
extent of labeling
3-8 mg double-stranded calf thymus DNA/g
storage temp.
−20°C
General description
Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and the linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.[1]
Application
Deoxyribonucleic acid−cellulose double-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.
Analysis Note
May contain a trace of Tris and EDTA.
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This Item | |||
|---|---|---|---|
| biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine pancreas |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| extent of labeling 3-8 mg double-stranded calf thymus DNA/g | extent of labeling ≥3 mg single-stranded calf thymus DNA/g | extent of labeling - | extent of labeling - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Can it be used to evaluate nucleosides from microorganisms? Example: thermonuclease in S. aureus
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Do you have recommendations for how to swell the DNA-cellulose and prepare it for subsequent use with DNA-binding proteins?
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This product may be swelled in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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How to dissolve this product to coat elisa plate, please?
1 answer-
This product is not tested for the suitability with coating ELISA plates. It may be helpful to read through the peer-reviewed articles associated with this product: https://www.sigmaaldrich.com/search/d8515?focus=papers&page=1&perpage=30&sort=relevance&term=d8515&type=citation_search
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How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?
1 answer-
A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.
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What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?
1 answer-
DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9). One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.
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Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?
1 answer-
We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)
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Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?
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DNA-Cellulose is stable in up to 5M NaCl. DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.
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How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?
1 answer-
DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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