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D2178

Sigma-Aldrich

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse

clone DAPK-55, ascites fluid

Synonym(s):

Anti-DAP Kinase

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About This Item

MDL number:
MFCD00282220
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DAPK-55, monoclonal

mol wt

antigen 160 kDa

contains

15 mM sodium azide

species reactivity

human, monkey

technique(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:250 using COS-7 cell extract

isotype

IgG1

UniProt accession no.

P51397

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DAP(1611)

General description

Monoclonal Anti-Death Associated Protein (DAP) Kinase (mouse IgG1 isotype) is derived from the DAPK-55 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Death-associated protein 1 (DAP1) gene is mapped to human chromosome 9q34.1. It belongs to the DAP family.
The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain.

Immunogen

recombinant human DAP kinase expressed in bacteria.

Application

Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.
Death-associated protein 1 (DAP1) participates in the regulation of cell growth and death including that of cancer cells. DAP-kinase has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Death-associated protein kinase increases glycolytic rate through binding and activation of pyruvate kinase
Mor I, et al.
Oncogene, 31(6), 683-683 (2012)
The role of DAPK-BimEL pathway in neuronal death induced by oxygen-glucose deprivation
He C, et al.
Neuroscience, 258(6), 254-262 (2014)
H Y Chen et al.
Oncogene, 34(40), 5141-5151 (2015-01-27)
Cullin 3 (Cul3)-family ubiquitin ligases use the BTB-domain-containing proteins for the recruitment of substrates, but the regulation of this family of ubiquitin ligases has not been completely understood. KLHL20 is a BTB-family protein and targets tumor suppressor promyelocytic leukemia protein
KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination
Chen HY, et al.
Oncogene, 34(40), 5141-5141 (2015)
Death associated protein 1 is correlated with the clinical outcome of patients with colorectal cancer and has a role in the regulation of cell death
Jia Y, et al.
Oncology Reports, 31(1), 175-182 (2014)
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